Protein-Engineered Polymers Functionalized with Antimicrobial Peptides for the Development of Active Surfaces

Antibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being source these infections. This suggests need for new antimicrobial biomaterial designs able withstand pressure resistance. Recombinant protein polymers (rPPs) are an emerging class nature-inspired biopolymers unique chemical, physical and biological properties. These can be functionalized molecules utilizing recombinant DNA technology then produced in microbial cell factories. In this work, we report functionalization rPBPs based on elastin silk-elastin different peptides (AMPs). were Escherichia coli, successfully purified employing non-chromatographic processes, used production free-standing films. The activity materials was evaluated against Gram-positive Gram-negative bacteria, results showed that demonstrated activity, pointing out potential development advanced materials.